Open AccessDetermination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration
Author(s) -
Jinfang Nie,
HaiLong Wu,
A-Lin Xia,
Shuijin Zhu,
Ying-Chao Bian,
Shufang Li,
RuQin Yu
Publication year - 2007
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.23.1377
Subject(s) - chemistry , sulpiride , detection limit , chromatography , matrix (chemical analysis) , fluorescence , urine , calibration , analytical chemistry (journal) , excitation , selectivity , residue (chemistry) , biological system , mathematics , optics , statistics , biochemistry , physics , receptor , electrical engineering , antagonist , biology , engineering , catalysis
This paper proposes a new and effective approach for the quantitative analysis of sulpiride, a significant antipsychotic drug, in human urine samples by the incorporation of excitation-emission matrix (EEM) fluorescence and second-order calibration methodologies based on the alternating fitting residue (AFR) and self-weighted alternating trilinear decomposition (SWATLD) algorithms. With the application of a second-order advantage, the proposed strategy could be utilized for a direct concentration determination of sulpiride with a simple pretreatment step, even in the presence of serious natural fluorescent interferences. The average recoveries of sulpiride in complex urine samples by using AFR and SWATLD with an estimated component number of three were 101.2 +/- 2.1 and 94.4 +/- 0.7%, respectively. Moreover, the accuracy of the two algorithms was also evaluated through elliptical joint confidence region (EJCR) tests as well as the figures of merit, such as sensitivity (SEN), selectivity (SEL) and limit of detection (LOD). The experimental results demonstrated that both algorithms, as promising quantitative alternatives, have been satisfactorily applied to the determination of sulpiride in human urine, but the performance of AFR was slightly better than that of SWATLD.