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This paper describes a method for obtaining information that can contribute to individual identification from a single hair colored with a hair dye product using a combination of surface-enhanced Raman scattering (SERS) and X-ray fluorescence (XRF) analyses. SERS and XRF spectra of single hog hairs colored with several commercially available hair dye products were measured. SERS spectral patterns tended to be different depending on the hair dye products used for hair coloring. However, SERS spectral patterns of single hog hairs colored with different hair dye products that would produce similar types of dyes were similar. By performing XRF analysis, characteristic metallic elements originating from some hair dye products were detected. Therefore, XRF can contribute to identifying the difference among colored hairs that cannot be identified only by SERS. SERS and XRF analyses of a single shed hair can contribute to individual identification used in forensic science.

Surface-enhanced Raman Scattering and X-ray Fluorescence Analyses of a Single Hair Colored with a Hair Dye Product