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The separation properties of arsenic species was investigated using HPLC-ICP-MS with several commercially available fluorocarbon stationary phases and no ion-pair reagents in HPLC. One pentafluorophenyl column showed the highest potential for the separation of a larger number of arsenic species when using simple acid-based mobile phases. After modification of the operational parameters in HPLC, the speciation analysis of 13 representative arsenic species: arsenite, arsenate, methylarsonic acid, dimethylarsinic acid, trimethylarsine oxide, tetramethylarsonium, arsenobetaine, arsenocholine, thio-dimethylarsinic acid, oxo-arsenosugar-glycerol, oxo-arsenosugar-phosphate, oxo-arsenosugar-sulfonate, and oxo-arsenosugar-sulfate, was achieved by HPLC-ICP-MS with the column along with a mobile phase of 0.05% heptafluorobutyric acid-methanol (99:1, volume per volume).

A Simple and Effective Method for Speciation Analysis of 13 Arsenic Species Using HPLC on a Fluorocarbon Stationary Phase Coupled to ICP-MS