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Natural sanguinarine (SG) was first used as a fluorescent probe to develop a novel ratiometric sensor for selective HSO 3  - detection. The nucleophilic addition reaction of HSO 3  - occurs at the C=N + group of SG, and subsequent breakage of the conjugated π cycle leads to a decrease in the SG iminium fluorescence that is accompanied by an increase in the alkanolamine fluorescence. Therefore, a ratiometric fluorescence method with a large wavelength shift can be established for HSO 3  - detection. Furthermore, cucurbit[8]uril was used as an efficient host to encapsulate SG for an improved selectivity for HSO 3  - detection over H 2 S. Our method benefits include little interference from other common anions and cations for HSO 3  - detection, suggesting a promising application in real sample analysis. Besides sensor development, the interaction of the natural SG with HSO 3  - was first demonstrated in this work to further get an insight into SG's pharmacology.

A Fluorescently Ratiometric Natural Probe for Selective Detection of Sulfur Dioxide Derivative and Host-Guest Supramolecular Regulation