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We developed a reference material (RM) for the determination of hexavalent chromium (Cr(VI)) in tap water. The tap water RM was prepared by adding a Cr(VI) standard solution to the raw material without acidification, i.e., under the original pH conditions of 7.6, because the decrease in the concentration of Cr(VI) was observed when the tap water had been adjusted to pH 1 with HNO 3 . The prepared tap-water RM (2 L) was packed in 10 fluororesin (PFA) bottles with an inside plug (200 mL each). Each PFA bottle (Cr(VI)-containing tap water) was sealed in a reclosable poly bag and then stored at 5°C in a refrigerator. The tap water RM had a Cr(VI) concentration of 51 μg L -1 . The concentration of Cr(VI) was determined by diphenylcarbazide absorptiometry using a 100-mm quartz cell. The detection limit of Cr(VI) in the sample solution corresponding to three-times the standard deviation (n = 5) of blank values was 0.51 μg L -1 . The homogeneity of Cr(VI) in the tap water RM was evaluated by an analysis of the variance after the Cochran test. There was no significant difference between the within-bottle and between-bottle variances of the analytical results, indicating that the tap water RM was sufficiently homogeneous. The stability of Cr(VI) in the tap water RM was investigated by monitoring the Cr(VI) concentration over a period of 6 months. The slope of the regression line of the Cr(VI) concentration versus the storage time did not significantly differ from zero, indicating that the tap water RM was stable for 6 months. The concentrations (50 - 51 μg L -1 ) of Cr(VI) in the tap water RM were in good agreement with the total chromium concentrations (50 - 51 μg L -1 ) obtained by atomic absorption spectrometry.

Preparation of a Reference Material for the Determination of Hexavalent Chromium in Tap Water