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A method is presented for the detection of a trace amount of cyanocobalamin (vitamin B 12 ) using a combination of solid-phase extraction and the measurement of cobalt in vitamin B 12 by a portable total-reflection X-ray fluorescence spectrometer. Using this combination, a detection limit of 1 ng/mL was achieved for vitamin B 12 .

Trace Determination of Cyanocobalamin (Vitamin B12) by Analyzing Cobalt Using a Portable Total-Reflection X-ray Fluorescence Spectrometer