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The major catechins in green tea leaves (Camellia sinensis L.) are (–)-epigallocatechin 3-O-gallate (EGCG), (–)epigallocatechin (EGC), (–)-epicatechin 3-O-gallate (ECG), and (–)-epicatechin (EC), which normally comprise more than 75% of tea polyphenols. Various biological and pharmacological properties of tea catechins have been reported. In particular, EGCG is known to exhibit more potent beneficial effects than the other catechins, including antioxidative,1,2 antimutagenic,3 antimutagenic/anticarcinogenic,4–6 antiallergenic,7,8 and antiatherosclerotic properties.9,10 Many published reports describe the determination and quantification of catechins. They can be classified into four methodological groups based on ultra violet,11–13 fluorescence,13,14 electrochemical,13–16 and chemiluminescence detection.9,17 Ultra violet and native fluorescence detection are relatively insensitive while electrochemical and chemiluminescence detection are more sensitive. 1,2-Diphenylethylenediamine (DPE) has been reported to react in a neutral medium with catechol compounds in the presence of hexacyanoferrate(III) to produce fluorescence intensity.18 We noticed that catechins include a catechol or pyrogallol group in their structures. Therefore, we used catechins [(+)-catechin (CA), EC, EGC, ECG, and EGCG] to establish suitable reaction conditions for a general analytical method.

Spectrofluorometric Determination of Catechins with 1,2-Diphenylethylenediamine