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The Cu(II)-catalyzed oxidation of 2,6-dichlorophenolindophenol (DCPI) in the reduced form by hydrogen peroxide was exploited for the development of a flow injection procedure for copper determination in plant digests and natural waters. For selectivity enhancement, a Chelex-100 resin mini-column (50 - 100 mesh) was incorporated to the manifold. The proposed system is very stable, handles about 60 samples per hour, yields precise results (RSD<2% within 5.0 and 70.0 μg l-1 Cu) and requires only 0.87 mg DCPI per measurement. Beer’s law is followed up to about 100 μg l-1 Cu and the detection limit is 0.09 μg l-1 Cu (in the injectate). Baseline drift was not observed during extended (5 h) operation periods. Results were in agreement with certified values of standard reference materials and recoveries within 95 and 104% were found.

A Flow Injection System for Spectrophotometric Catalytic Determination of Copper in Plant Digests and Natural Waters