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The fluorescence emission from fluoroquinolones is shown to be associated with the substituted quinoline portion of the molecule. Neutral, anionic, zwitterionic and cationic forms of the fluorophore are proposed to account for the fluorescent behavior in aqueous and organic media of different acidities. In aqueous solution a ground-state pKa was observed for NFX and CPFX from a study of the pH vs. fluorescence profile with excitation at 331 nm. The ground-state microscopic dissociation constants for NFX or CPFX were determined, explained the causes of fluorescence changing. The optimum conditions for the fluorimetric determination of NFX, CPFX are an acidic medium (pH=2-4.0) with λ ex =280nm or 331nm and λ em =445nm.

Fluorescence Investigation of Some Fluoroquinolones in Various Media: Ground-State Acid-Base Equilibra.