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Microdialysis is a sampling technique for in vivo analysis. In many cases, the dialyzate collected by a microdialysis probe is assayed by LC'-4, but the time resolution of such an assay is not sufficient for real-time monitoring. Recently, a few attempts have been made to develop an on-line sensing system based on the use of a microdialysis probe in combination with an enzyme electrode5,6 or an immobilized enzyme reactor.7'8 Such a system makes possible on-line monitoring of metabolism in living tissue. These attempts are well suited for the assay of an analyte in the dialyzate with complex matrixes, because an enzyme can recognize specifically the analyte of interest in the dialyzate. However, such a flow system is not strictly a real-time monitoring process, because of the delay time between sampling and detection. In this research, we prepared a microdialysis enzyme electrode which makes possible real-time and in vivo monitoring. The electrode consists of a platinum fiber working electrode and a silver/silver chloride fiber reference electrode inserted into the tip and in the upper compartment of the dialysis fiber. We also selected glucose electrodes using glucose oxidase as a typical model of the sensor of this type. The measurements were first done by holding stationary a soluble enzyme solution in the probe cavity, and then by the electrode with enzyme layer immobilized on the platinum fiber working electrode. The response characteristics of both electrodes are evaluated below.

Microdialysis Fiber Electrodes for Glucose with Soluble and Immobilized Glucose Oxidase