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2-(5-Bromo-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino)phenol (5-Br-PAPS) is one of the most useful spectrophotometric reagents for transition metals in pyridylazo compounds. In connection with the HPLC of metal complexes, 5-Br-PAPS is also excellent as a chelating agent owing to the sensitivity and thermodynamic stability of its complexes of metals, such as V, Cr, Fe, Co, Ni, and Cu.

analytical sciences

Simultaneous Determination of Copper, Iron and Nickel by High-Performance Liquid Chromatography after Preconcentration with Extraction and Back-Extraction