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We reported2 on a new method for the determination of hydrogen peroxide (denoted by H202) based on a fading of an o-sulfophenylfluorone (denoted by SPF)titanium(IV) complex in the presence of ethylenediaminetetraacetic acid (denoted by EDTA). Although this method is highly sensitive, since the apparent molar absorptivity is 2.3X1051 mol-1 cm', a heating procedure for a long time is necessary for a maximum color development. In a continuation of this investigation of an H2O2 determination, an attempt was made to obtained an o-hydroxyhydroquinonephthalein (denoted by QP)titanium(IV) complex using QP instead of SPF so as to improve the QP-titanium(IV) complex method. This paper deals with a simple and rapid procedure for determination of H202 by utilizing the QP-titanium(IV) complex. The proposed method was applied to an H2O2 determination in rain water. In addition, this method was also applied to assays of glucose and uric acid in human urine by combined use of an appropriate enzyme to produce H2O2 through enzymatic oxidation.

Spectrophotometric Determination of Hydrogen Peroxide Using o-Hydroxyhydroquinonephthalein, Titanium(IV) and Ethylenediaminetetraacetic Acid