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ABSTRAK Kelapa Dalam Mapanget (DMT) merupakan salah satu kelapadalam unggul produksi dan kadar minyak serta protein yang baik.Beberapa populasi generasi DMT telah diseleksi selama tahun 1957 –1979 menghasilkan populasi DMT 32. Penelitian ini bertujuan untukmelacak tetua melalui aliran gen dalam beberapa generasi populasi kelapaDMT-32 hasil penyerbukan campuran polen, pada taraf DNA berdasarkanpenanda mikrosatelit (SSR). Bahan tanaman yang digunakan dalampenelitian ini adalah populasi hasil penyerbukan kelapa DMT-32 generasikedua (DMT-32 S2), populasi DMT-32 generasi ketiga (DMT-32 S3), danpopulasi DMT-32 generasi keempat (DMT-32 S4) berturut-turut sebanyak9, 40, dan 38 pohon. Analisis hubungan tetua dengan zuriatnya meng-gunakan program komputer CERVUS ver. 2.0. Jumlah primer SSR yangdigunakan sebanyak 19 primer dan 15 di antaranya dapat digunakan untukmelacak tetua dari individu-individu kelapa DMT-32 S3 dan DMT-32 S4.Semua individu DMT-32 S2 menjadi tetua dari individu-individu DMT-32S3, tetapi tidak semua individu DMT-32 S3 menjadi tetua dari DMT-32S4. Hasil pelacakan tetua menunjukkan bahwa 2 pohon DMT-32 S3 yangbenar-benar hasil penyerbukan hasil zigot polen sendiri dari satu pohonkelapa DMT-32 S2 No.8, dan 1 pohon zuriat dari DMT-32 S2 No.3. PadaDMT-32 S4 ada 2 individu pohon yang benar-benar merupakan hasilpenyerbukan zigot polen sendiri pohon DMT-32 S3 No.28, masing-masing1 pohon zuriat dari DMT-32 S3 No.32 dan DMT-32 S3 No.35. DMT-32S2 No.1 merupakan tetua dari 8 individu DMT-32 S3, dan lima darizuriatnya adalah tetua dari 13 individu DMT-32 S4. DMT-32 S2 No. 2adalah tetua dari 9 individu DMT-32 S3 dan empat nomor di antaranyamenjadi tetua dari 14 individu DMT-32 S4. DMT-32 S2 No.3 merupakantetua dari 11 individu DMT-32 S3 dan enam nomor pohon di antaranyamenjadi tetua dari 18 individu DMT-32 S4. DMT-32 S2 No.4 memiliki 5zuriat dan dua nomor pohon di antaranya menjadi tetua dari 7 individuDMT-32 S4. DMT-32 S2 No.5 merupakan tetua dari 10 pohon DMT-32S3 dan enam nomor pohon di antaranya menjadi tetua dari 24 pohonDMT-32 S4. DMT-32 S2 No.6 adalah tetua dari 4 zuriat DMT-32 S3 danhanya satu nomor pohon yang menjadi tetua dari 4 individu pohon DMT-32 S4. DMT-32 S2 No.7 merupakan tetua dari 10 zuriat pohon DMT-32S3, lima di antaranya merupakan tetua dari 20 pohon DMT-32 S4. DMT-32 S2 No.8 memiliki 12 zuriat DMT-32 S3, dan empat di antaranyaadalah tetua dari 15 pohon DMT-32 S4. DMT-32 S2 nomor 9 merupakantetua dari 7 pohon DMT-32 S3, dan empat diantaranya adalah tetua dari17 pohon DMT-32 S4.Kata kunci : Cocos nucifera L, Mapanget Tall Coconut (DMT 32),mikrosatelit, SSR, pelacakan tetua ABSTRACT Parentage analysis of Mapanget Tall Coconut No.32 (DMT-32)population via gene flow based on Microsatellite Markers (SSR)Mapanget Tall Coconut (DMT) is one of the superior coconut for itsproduction, coconut oil and protein. Several generation of the DMTpopulation has been selected in 1957 – 1979 producing DMT 32generations. The aim of this research was to analyze the parents ofMapanget Tall Coconut No.32 (DMT-32) in DNA level via gene flowbased on microsatellite markers (SSR). Plant materials used in thisresearch were nine (9) palms of DMT-32 S2, 40 palms of DMT-32 S3 and38 palms of DMT-32 S4. Relationship between parents and progeny wereanalyzed by using CERVUS ver. 2.0 computer program. Among 19 SSRprimers used, 15 of them can be used in parentage analysis of MapangetTall Coconut No.32 of third and fourth generations. All of 9 (nine) palmsof DMT-32 S2 are the parents of DMT-32 S3, but some of those palms ofDMT-32 S3 are not the parents of DMT-32 S4. The result of parentageanalysis showed that two palms of DMT-32 S3 were progeny of selfedDMT-32 S2 No.8, and one palm was progeny of selfed DMT-32 S2 No.3.In DMT-32 S4 there were two palms progeny of DMT-32 S3 No.28 andone palm was progeny of DMT-32 S3 No.32 and DMT-32 S3 No.35respectively. DMT-32 S2 No.1 had 8 progeny in DMT-32 S3 and five ofthose were the parents of 13 individu DMT-32 S4. DMT-32 S2 No. 2 had9 progeny in DMT-32 S3 and four of those were the parents of 14 individuDMT-32 S4. DMT-32 S2 No.3 had11 progeny in DMT-32 S3 and six ofthose were the parents of 18 individu DMT-32 S4. DMT-32 S2 No.4 had 5progeny in DMT-32 S3 and two of those were the parents of 7 individuDMT-32 S4. DMT-32 S2 No.5 had 10 progeny in DMT-32 S3 and six ofthose were the parents of 24 individu DMT-32 S4. DMT-32 S2 No.6 had 4progeny in DMT-32 S3 and only one was the parent of 4 individu DMT-32S4. DMT-32 S2 No.7 had 10 progeny in DMT-32 S3 and five of thosewere the parents of 20 individu DMT-32 S4. DMT-32 S2 No.8 had 12progeny in DMT-32 S3 and four of those were the parents of 15 individuDMT-32 S4. DMT-32 S2 No.9 had 7 progeny in DMT-32 S3 and four ofthose were the parents of 17 individu DMT-32 S4.Key words : Cocos nucifera L, Mapanget Tall Coconut (DMT-32),microsatellite, SSR, parentage analysis

PELACAKAN TETUA POPULASI KELAPA DALAM MAPANGET No.32 (DMT-32) MENGGUNAKAN ANALISIS ALIRAN GEN (Gene Flow) BERDASARKAN PENANDA MIKROSATELIT (SSR)