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A fluorescence spectroscopy technique has been widely used for characterizing the fluorescence of nanomaterials. The technique provides fast and sensitive measurements that are applicable in many research and industrial areas. Obtained absorbance, excitation, and fluorescence emission spectra can be used to determine sample concentrations, binding kinetics of biomolecules, reaction mechanisms, and many more applications. This scientific operating procedure SOP provides general guidance for using fluorescence spectroscopy techniques with an instrument such as a HORIBA Scientific NanoLog® spectrofluorometer to determine fluorescence emission and UV-Vis-NIR absorbance of nanomaterials in solution. Procedures and recommendations of sample preparation, instrument preparation, sample analysis, and results analysis are briefly described. The procedure was tested on a variety of nanomaterial solutions. DISCLAIMER: The contents of this report are not to be used for advertising, publication, or promotional purposes. Citation of trade names does not constitute an official endorsement or approval of the use of such commercial products. All product names and trademarks cited are the property of their respective owners. The findings of this report are not to be construed as an official Department of the Army position unless so designated by other authorized documents. DESTROY THIS REPORT WHEN NO LONGER NEEDED. DO NOT RETURN IT TO THE ORIGINATOR.

Determination of fluorescence emission and UV-Vis-NIR absorbance for nanomaterials solution using a HORIBA Scientific NanoLog® spectrofluorometer