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This scientific operating procedure (SOP) provides a standard method for assessing the aquatic toxicity of insensitive munitions (IM) compounds using the freshwater amphipod Hyalella azteca. The endpoints for the 10day static-renewal test are survival and growth. The 10-day test is conducted at 23 ± 1 °C in 300 mL glass beakers containing 200 mL of test solution. Juvenile amphipods are exposed to a decreasing concentration series of IM compounds (e.g., 100%, 50%, 25%, 12.5%, and 6% of the highest concentration) and to a negative control. For each concentration, eight replicate beakers, each containing ten H. azteca are used. Test solutions are renewed three times during the test. Water quality parameters are measured at the start, daily during the exposure period and at test termination. The tests are only valid if the mean survival for the control treatment is 80% or greater. Instructions and requirements are provided for handling H. azteca before and during the toxicity test, preparing aqueous mixtures, initiating and terminating 10-d tests, maintaining appropriate test conditions, making necessary observations and water quality measurements, assessing the survival and growth endpoints and obtaining samples for chemical analysis. DISCLAIMER: The contents of this report are not to be used for advertising, publication, or promotional purposes. Citation of trade names does not constitute an official endorsement or approval of the use of such commercial products. All product names and trademarks cited are the property of their respective owners. The findings of this report are not to be construed as an official Department of the Army position unless so designated by other authorized documents. DESTROY THIS REPORT WHEN NO LONGER NEEDED. DO NOT RETURN IT TO THE ORIGINATOR.

Assessing the Aquatic Toxicity of Insensitive Munitions (IM) Compounds Using 10-Day Aqueous Exposures with the Amphipod Hyalella Azteca : Scientific Operating Procedure Series : Characterization of IMX Ecotoxicological Effects