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The objective of this study was to estimate the genotype and allele frequencies of the variants A1 and A2 of the b-casein and determine their relationship with milk production per lactation (PL305) in the Tropical Milking Criollo bovine (CLT). Blood samples were collected from 151 females; from DNA a segment of the b-casein gene was amplified by PCR and genotyped by digestion with restriction endonuclease. Genotype frequencies for A1A1, A1A2 and A2A2 were 0.09, 0.78 and 0.13 (p£0.05) and allele frequencies for A1 y A2 were 0.48 y 0.52 (p>0.05). Genotypic and allelic frequencies of b-casein of the CLT cattle were similar to the Holstein breed. No b-casein genotypes effect was observed in PL305 (p>0.05); least squares means were 1124±93, 1130±152 and 1257±74 for A1A1, A1A2 y A2A2. They will continue simultaneous studies of genotypes of b-casein, k-casein and b-lactoglobulin and production and composition of milk of CLT cows.

Frecuencias genotípicas y alélicas de la b-caseína en el bovino Criollo Lechero Tropical de México