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The effect of adding different levels of vitamin C to semen extenders on cryosurvival of spermatozoa obtained from West African Dwarf (WAD) bucks during cryopreservation was studied. Tris-egg yolk based extenders supplemented with 2, 4, 6 and 8 m M of vitamin C were diluted with pooled semen samples. The diluted semen samples were cryopreserved for 30 days and thereafter evaluated for sperm quality characteristics. Following cryopreservation, acrosome reaction and capacitation of spermatozoa were induced in vitro. The results showed higher (P<0.05) sperm motility, acrosome and membrane integrities in extenders supplemented with vitamin C compared to the control and highest values were recorded in 8 mM. Reduced concentrations of MDA were observed in extenders supplemented with 6 mM and 8 mM of vitamin C compared to other levels and the control. The extenders supplemented with vitamin C had higher (P<0.05) values of arginase activity compared to the control. Lower (P<0.05) leukocytes were observed in extenders supplemented with vitamin C compared to the control and lowest values (P<0.05) were observed by the addition of 8 mM. The results showed that more spermatozoa cryopreserved in extenders supplemented with 6 mM vitamin C underwent acrosome reaction and capacitation (P<0.05). The finding indicated that vitamin C improved sperm quality parameters. The improvement in viability parameters of cryopreserved WAD buck spermatozoa was optimum at 8 mM of vitamin C.

Supervivencia de espermatozoides de cabra crioconservados en diluyente a base de yema de huevo-tris suplementado con vitamina C