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Simplified hematoxylin and eosin (H&amp;E) staining followed by cryo-sectioning enables rapid identification of cancerous tissue within the procedure room during Mohs micrographic surgery. Yet, a faster evaluation method is desirable as the staining protocol requires physically sectioning of the tissue after freezing, which leads to prolonged sectioning time along with the frozen artifacts that may occur in frozen sectioning.

Rapid tissue histology using multichannel confocal fluorescence microscopy with focus tracking