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Sarcoma & Connective Tissue Oncology Program & Department of Orthopaedic Surgery, Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA; Department of Surgery, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA; Center for Imaging Medicine, Thayer School of Engineering, Dartmouth College, Hanover, NH USA; Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA Contributions: (I) Conception and design: All authors; (II) Administrative support: None; (III) Provision of study materials or patients: None; (IV) Collection and assembly of data: All authors; (V) Data analysis and interpretation: All authors; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. These authors contributed equally to this work. Correspondence to: Eric R. Henderson, MD. Sarcoma & Connective Tissue Oncology, Department of Orthopaedic Surgery, Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA. Email: eric.r.henderson@gmail.com.

Osteosarcoma mineralization changes on radiographs have moderate correlation to chemotherapy response using bone subtraction methodology