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Pollution from industrial effluents and anthropogenic sources is becoming a very big ecological problem in Nigeria. A typical example of such pollutants is cyanide which can be degraded to a less toxic compound by some microorganism. Some facultative anaerobic bacteria such as Bacillus licheniformis utilizes cyanide as a substrate for survival in industrial contaminated and polluted ecosystem.  This study screened for bacterial from industrial effluents in Ilorin that can synthesize rhodanese, an enzyme that can degrade cyanide to less toxic substance. Bacillus licheniformis was isolated and biochemically characterized. Rhodanese was partially purified using 85% ammonium sulphate precipitation, ion exchange chromatography on Sephadex CM-50. The molecular weight of the enzyme was determined on a gel filtration column (Sephadex G-100) and finally on Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The kinetic parameters (K m and V max ) were determined. Statistical analysis was performed using one-way analysis of variance and results were considered significant at p 0.05). The high level of expression of rhodanese in B. licheniformis suggests that the enzyme possess functional cyanide detoxification mechanism, a bioremediation process for the survival of both plants and animals in the environment. Microbial enhancement of this kind is recommended to help in reduction of toxic materials from industrial effluents.

Isolation and Characterization of Rhodanese from Bacillus licheniformis Obtained from Industrial Effluents of Steel Rolling Industry in Ilorin