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In this work, a three-dimensional porous poly(dopamine)/graphene-modified electrode was prepared. The modified electrode was characterized by scanning electron microscopy and electrochemical methods. The electrochemical behaviours of guanine and adenine with the modified electrode were investigated by cyclic voltammetry. The experimental results showed that the modified electrode had good electrocatalytic ability for the oxidation of guanine and adenine. Differential pulse voltammetry was used to simultaneously detect guanine and adenine in 0.1 mol/L PBS (pH 7.0). The oxidation peak currents of guanine and adenine were linear with their concentrations in a range of 1.0-58 μmol/L. The linear regression equation for guanine was ip = -1.95010.0991c (R 2 = -0.9973) with a detection limit of 0.34 μmoL (S/N = 3), and the linear equation for adenine was ip = -1.20050.1049c (R 2 = -0.9943) with a detection limit of 0.31 μmoL (S/N = 3). In addition, this modified electrode exhibited favourable stability, reproducibility and good anti-interference ability.

international journal of electrochemical science

Electrochemical Behaviours of Guanine and Adenine and their Simultaneous Determination using a Three-Dimensional Porous Poly(dopamine)/Reduced Graphene Oxide-Modified Electrode