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In this study, we synthesized graphene through a green approach in which consuming of the chemical materials in production process decreased and reached to the lowest value. Bovine Serum Albumin (BSA) was used as the stabilizer for exfoliated graphene sheets. Synthesis of the functionalized graphene was confirmed by uv-vis spectroscopy. The physical and chemical properties of the synthesized graphene were compared with conventional graphene oxide. Atomic force microcopy (AFM) was utilized to the evaluating the thickness of graphene sheets. Functional groups of graphene were determined by FTIR spectroscopy and Raman spectra supported the AFM and FTIR results. Main electrochemical properties of the graphene were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). In the following, a biosensing application was investigated to determine ascorbic acid (AA) via a non-enzymatic interaction between the graphene and AA. The present graphene-modified BSA (G-BSA) based sensor provided a favorable opportunity for the design of non-enzymatic electrochemical sensors with extraordinary performance via higher some sensing characteristics and simple fabrication.

A Novel Approach to Facile Synthesis and Biosensing of the Protein-Regulated Graphene