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The purpose of this study is to explore a novel, sensitive, simple and convenient method for the detection of lappaconitine. Carbon nanotube fibers were fabricated by a coaxial electrospinning fiber technique and established the base of an electrostatic spinning carbon nanotube fiber modified glassy carbon electrode (GCE/ESCNF). The electrochemical characteristics of lappaconitine in different conditions (concentration, pH value, scan rate, etc.) at the GCE/ESCNF were investigated by cyclic voltammetry. The results showed that the modified electrodes exhibited excellent electrocatalytic sensitivity towards the electrochemical detection of lappaconitine. When the pH of the BrittonRobinson (B-R) buffer solution was 5.8, aconitum alkaloid lappaconitine gave a well-shaped oxidation peak in cyclic voltammetry (CV). The oxidation peak ranged from -0.02 V to +0.03 V (vs. SCE). This phenomenon illustrates that an irreversible redox reaction of lappaconitine occurred on the electrostatic spinning carbon nanotube fiber modified electrodes. When the density of lappaconitine was between 1.2 to 74 μg/mL, the peak current and lappaconitine concentration had a linear relationship. The detection limit was 0.884 μg/mL. The fabricated GCE/ESCNF had well good reproducibility and stability. This method is also applicable to the detection of other aconitum lappaconitine alkaloids with an average recovery rate of 100.74% and a relative standard deviation of 1.86%.

A Novel Strategy for the Determination of Lappaconitine with Use of Electrostatic Spinning Carbon Nanotube Fiber Modified Glassy Carbon Electrode