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Large use of pesticide is an important issue in modern agriculture. Development of a reliable analytical method for pesticide detection in essential for many fields. In this work, we proposed an electrochemical based detection method for methyl parathion, an organophosphate pesticide, residue in the Fritillaria thunbergii, a medicinal plant has been cultivated for several thousand years, based on the acetylcholinesterase immobilized gold nanoshpere (Au NS) modified electrode. The combination of acetylcholinesterase and Au NS displayed a strong synergetic effect on enhancing the detection properties of methyl parathion. Au NS could catalyze the oxidation process of the enzymatically produced thiocholine and result a high sensitivity. After optimization, the proposed biosensor was used for analyzing the methyl parathion residue in freshly harvested Fritillaria thunbergii.

Eelectrochemical Detection of Methyl Parathion in Fritillaria thunbergii Based on Acetylcholinesterase Immobilized Gold Nanoshpere