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Keyworks histidine amino acid supplementation alcoholic fermentation S. cerevisiae Amino acid supplementation in alcoholic fermentation of molasses must and sugarcane syrup using CAT1 industrial lineage. Nitrogen supplementation may contribute to higher tolerance of S. cerevisiae strains to the different deleterious conditions, such as those that are faced in the Brazilian ethanol production. The present study evaluated the influence of amino acid supplementation under the growth and cell viability of CAT-1 industrial strain under conditions of ethanolic and osmotic stress (YNB medium with 10 and 12% v/v ethanol and molasses must with 15, 20, 25 and 30% ART). Amino acid supplementation was also evaluated in fermentations of sugarcane molasses and syrup, using cell recycle. The results revealed that amino acid supplementation caused distinct effects on the physiological behavior of the yeast according to the medium/musts provided. Histidine supplementation favored the CAT-1 for higher growth and cell viability in molasses and syrup musts. The results showed that the supplementation of 200 mg.L of amino acid can favor or degrade the growth and viability of the CAT-1 in fermentations simulating Brazilian industrial conditions. Supplementation with histidine proved to be the most promising for increasing the tolerance of the industrial strain.

Suplementação de aminoácidos na fermentação alcoólica de mostos de me-laço e xarope de cana-de-açúcar empregando linhagem industrial CAT-1