Open AccessTransient expression of <em>uid</em>A gene in grapevine protoplasts after PEG-mediated transformation
Author(s) -
Rahma Jardak,
Ahmed Mliki,
Abdelwahed Ghorbel,
Götz M. Reustle
Publication year - 2002
Publication title -
oeno one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.516
H-Index - 13
ISSN - 2494-1271
DOI - 10.20870/oeno-one.2002.36.2.975
Subject(s) - protoplast , transformation (genetics) , microbiology and biotechnology , kanamycin , agrobacterium , biology , peg ratio , gene expression , gene , chemistry , botany , biochemistry , finance , economics
Leaf protoplasts, isolated from Vitis vinifera «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of Vitis sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which carries the β-glucuronidase (gus) and the neomycin phosphotransferase II (nptII) genes. The treated protoplasts were cultivated in CPW13 medium without kanamycin selection. 48h after the PEG transformation, transient expression of gus gene was detected histochemically and fluorimetrically in the protoplast cultures.
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