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A continuous oral administration of N-nitrosobutylurea (NBU) for 6 months induced a variety of tumors besides mammary tumors in variously conditioned Wistar/Furth (W/Fu) rats of both sexes. The animals were castrated before the NBU treatment or castrated and supplemented with biweekly injections of 0.4mg of progesterone and 0.01mg of estradiol benzoate or with an ovarian graft under the kidney capsule. Leukemias of myeloid and undifferentiated-cell types developed in 7 rats with a

Induction of tumors in the brain, kidney, and other extra-mammary gland organs by a continuous oral administration of N-nitrosobutylurea in Wistar-Furth rats.