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Mice neonatally grafted with glandular stomach were continuously given Nmethyl-N'-nitro-N-nitrosoguanidine (NG) dissolved in drinking water (50mg/L) for 10 months. This dose of NG did not induce gastric carcinoma, but gave rise to leiomyosarcomas in the wall of the gastric cysts in the subcutaneous tissue, in 5 out of 26 mice. Atypical hyperplasia of epithelial cells in the cysts was also found in 2 mice. A strengthened and remote action of this carcinogen in gastric cysts was suggested.

Leiomyosarcomas induced by oral administration of N-methyl-N'-nitro-N-nitrosoguanidine in gastric cysts grafted in subcutaneous tissue of mice.