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In this series of experiments the influence of oxygen at high pressure (3 atmospheres absolute) on the growth of cells was investigated, using HeLa, FL, and Yoshida sarcoma cells. The inhibitory effect of oxygen at high pressure on the propagation of cells was considerably severe and highly significant compared with those on other groups, which were exposed to oxygen at atmospheric pressure, air at 3 atmospheres absolute, and air at atmospheric pressure. The relationships among the experimental groups exposed to various conditions of oxygen treatment for 24 hours were of great interest. The results obtained here indicate that the influence of oxygen at high pressure on the proliferation of Yoshida sarcoma cells is more severe than that on two established cell lines.

Influence of oxygen at high pressure on the proliferation of cells in tissue culture.