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The result of the investigation revealed that there were significant variations in germination and seedling growth of custard apple due to cow urine concentrations with soaked duration and PGPR inoculation and interaction of three factors. amongst the different cow urine concentration G1 (20%) recorded earliest germination (22.3 days), 50 % germination (40.2 days) % of germination at 30 days ( 43.5 %), percent of germination at 60 days ( 70.4 %), whereas S1 contributed to minimum days (22.3) to taken start germination, 50% germination (43.6days) and % of germination at 30 days (43.5%). At 60 days (61.3%). Growing media (PGPR), Contribute to early germination days taken to start germination, days taken to 50% germination, % of germination at 30 days% of germination at 60 days were better in 6% growing media under P3. Among the three factors cow urine was superior in respect to germination parameters. The height of shoot (5.5, 7.8, 12.2, 13.3 cm), no. of leaves (5.7, 6.6, 9.5, 11.0), girth of stem (1.6, 2.2, 2.5, 3.0) under G1. Maximum height of shoot (5.7, 8.6, 12.6, 14.5) was recorded among the three factors interaction G1S1P3. The seedling height (22.8 cm), no. of roots (32.0), fresh weight of shoot and dry weight of shoot (2.1 g and 0.64 g), fresh weight of root and dry weight of root (0.64 and 0.48 g), were maximum under (G1 ) 20 % cow urine. Speed of germination (0.68 seed/ day), coefficient velocity of germination (2.61) were maximum under G1P3 treatment combination. The leaf area index during 120-150 days (3.9), leaf area duration (1508.84 cm 2 days), energy interception (0.49) recorded under G1 (20% Cow urine). The maximum LTR was recorded under G0 (control).Seedling vigour index I (1075.66 cm), Seedling vigour II (58.83 G), % of survival (58%) was recorded and it was maximum under 20 % cow urine. While soaked duration contributed to (1072 cm), (50.16 g) under S1 (24hrs) soaked duration. The interaction between cow urine and soaked duration was superior among all the interaction height of shoot at various stages (4.4, 7.1, 12,6,13.9 cm), no. of leaves (5.5, 6.7, 9.2, 10.8), girth of stem (1.4, 2.1, 2.0, 2.7) Speed of germination, coefficient velocity of germination, were recorded under G1S1 treatment combination. Combine effect of cow urine and PGPR recorded no. of roots (28), height of seedling (21.24 cm), length of root (9.2 cm), days taken to start germination (22.41), % of germination at 30 and 60 days (51% and 83.3 %) under G1P3 it was superior over rest to cow urine with growing media treatments. Interaction among three factors was non-significant the parameters, days taken to 50% germination, fresh and dry weight of shoot, fresh weight of shoot, leaf area duration, leaf transmission ratio, speed of germination, coefficient velocity of germination energy interception. K e y w o r d s Seed germination, Pre sowing treatments, Custard apple Accepted: 15 October 2020 Available Online: 10 November 2020 Article Info Int.J.Curr.Microbiol.App.Sci (2020) 9(11): 1806-1818

Effect of Different Pre Sowing Treatments on Seed Germination and Growth of Custard Apple (Annona squamosa L.) Seedlings