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The present study was conducted to evaluate the effect of supplementation of glutathione and α-tocopherol in TRIS and skim milk based extenders on motility parameters of ram semen at refrigeration temperature. The research findings of the present study shown that, addition of glutathione improved motile sperm and reduced immotile sperm percentage than α-tocopherol in skim milk than TRIS egg yolk based extenders. Addition of glutathione improved rapid progressive sperm percentage than α-tocopherol in TRIS egg yolk than skim milk based extenders. Addition of αtocopherol in TRIS egg yolk reduced slow progressive sperm percentage, whereas improved in skim milk based extenders as compared to addition of glutathione. Addition of either glutathione or α-tocopherol did not reduce the non-progressive sperm percentage in skim milk and TRIS egg yolk based extenders. The volume of semen (Mean±SE) ranged from 0.83±0.04 to 0.94±0.03 mL having creamy colour. The mass activity of semen samples was with ++++ for all semen samples in NARI Suwarna strain of sheep. The addition of glutathione in skim milk based extender improved motile sperm percentage than TRIS egg yolk extender at 72 h of refrigeration. The addition of glutathione increased rapid progressive sperm percentage at 72 h of refrigeration in TRIS egg yolk than skim milk based extender. K e y w o r d s

international journal of current microbiology and applied sciences

Effect of Supplementation of Glutathione and α-Tocopherol in Tris and Skim Milk Based Extenders on Motility Parameters of Ram Semen at Refrigeration Temperature