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Bioassay helps in ascertaining the potency and relative toxicity of different insecticides. The bioassay methods commonly employed to insect toxicity evaluations are topical application by Potter’s tower, injection method, leaf dip, contact or residue film method etc. The LC50 values serve as a ready reckoner for the selection of insecticides to work out strategy for the management of insect pest under field conditions. Also, such baseline data would provide a record for detecting resistance level of the insect pests to various insecticides at different periods. The cotton bollworm H. armigera, continues to maintain a high pest status in India where Bioassay studies were conducted in the Department of Entomology, College of Agriculture, Rajendranagar, Hyderabad to determine the relative toxicity of selected novel insecticides viz., chlorfluazuron, chlorantraniliprole, spinetoram, indoxacarb, emamectin benzoate and spinosad against second instar larvae of H.armigera by topical application, residue method and surface diet method. The data on the toxicity of insecticides to H. armigera, clearly indicated that among the six insecticides tested against the second instar, irrespective of the method of application, spinetoram, indoxacarb and spinosad showed greater toxicity compared to chlorfluazuron, chlorantraniliprole and emamectin benzoate. While in the latter three insecticides, the toxicity to H. armigera was comparatively higher in topical application than other methods although in case of emamectin benzoate it was almost similar with surface diet method. K e y w o r d s

Bioassay Studies for Testing Toxicity of Novel Insecticides against Helicoverpa armigera (H.)