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The root knot nematodes (Meloidogyne spp.) are sedentary endoparasites and most damaging agricultural pests, attacking a wide range of vegetable crops. Apart from the economic loses; nematodes are also adversely affecting the quality of these crops. In India, An attempt was made to find out natural and cheaper methods for the control of vegetable pest. Locally available plant C. procera was used and a good methodology was developed to extract the secondary metabolic compounds from the latex. Secondary metabolic compounds extracted in form of different polarity fractions and in purity of >90% as single pure compound in each fraction, showed tremendous activity in vitro test against secondary stage juveniles of Meloidogyne javanica. The extracted fractions were found toxic to juveniles by different capabilities between concentrations ranged from 50-300 g ml -1 . The activity of compounds was found dependent on concentration, time of exposure and the polarity of the extracted fractions. Two highly lipophilic nature of fractions having clear solubility in solvents such as hexane, dichloromethane were found extremely active by giving larval mortality of test nematode by more than 90% at their 250 g ml -1

international journal of current microbiology and applied sciences

Isolation of Biocidal Compounds of Calotropis Latex and their in vitro and in vivo Effect on the Mortality of Root Knot Nematode (Meloidogyne javanica) in Brinjal and Chilli