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Piperine is chief bio-molecular active compound of Piper longum, exhibited various pharmacological activities. In addition it improves the bioavailability of other nutritive substances. The aim of the study was to induce callus from Piper longum, and compared the quantification of piperine in callus and wild grown Piper longum. The callus were produced by transferring the sterile leaves on MS medium containing different concentration of cytokinins like 0.5 mg/l BAP (6-benzylaminopurine), 1.0 mg/l KN (kinetin) with auxins like 0.5 2.0 mg/l 2,4-D (2 4-dichlorophenoxyacetic acid). The petroleum ether, ethanol and aqueous extracts of Piper longum plant and callus were prepared. The content of piperine in plant extract and callus extract were performed by HPLC. The phytochemical study uncovered the nearness of different secondary metabolites in various extract of plant. The HPLC chromatogram displayed that content of piperine present in callus extract was higher compared to field grown plants.

Comparative Analysis of Piperine in Wild Plant and Callus of Piper longum by HPLC Method