Open AccessThe first isolation of equine arteritis virus in Argentina
Author(s) -
María Gabriela Echeverría,
Marcelo Ricardo Ítalo Pecoraro,
Cecília Mônica Galosi,
María Elisa Etcheverrigaray,
Edgardo Omar Nosetto
Publication year - 2003
Publication title -
revue scientifique et technique de l oie
Language(s) - English
Resource type - Journals
eISSN - 1608-0637
pISSN - 0253-1933
DOI - 10.20506/rst.22.3.1458
Subject(s) - virology , semen , virus , biology , reverse transcription polymerase chain reaction , virus isolation , isolation (microbiology) , classical swine fever , polymerase chain reaction , arterivirus , microbiology and biotechnology , medicine , covid-19 , gene , infectious disease (medical specialty) , genetics , pathology , messenger rna , disease
This paper describes the first isolation of equine arteritis virus (EAV) in Argentina. The virus was isolated from the semen of an imported seropositive stallion held in isolation at a breeding farm in Tandil in the Buenos Aires Province. In addition, viral nucleic acid was detected in seminal plasma using the reverse-transcription polymerase chain reaction. The isolated virus was propagated in cell cultures and confirmed as EAV by indirect immunofluorescence and virus neutralisation, using a serum specific for the reference Bucyrus strain of EAV. As far as the authors are aware, this is the first time that EAV has been isolated in South America. The equine industry is very important for Argentina and international movement of horses is very intensive. This finding may have effects on the international trade of horses and semen from Argentina.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom