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Aeromonas  hydrophila  is  a  motile  Aeromonas  bacteria  that  cause  Septicemia  (MAS)  or  the disease kwon as red spots (Plumb, 1994). These bacteria attack the various types of freshwater fish such as African catfish, carp and prawns. Treatment of MAS is still a lot of use antibiotics, whereas antibiotic a lot  side  effects.  This  study  tries  for  find  an  alternative  use  antibacterial  substances  using  natural chemicals derived from medicinal plants of leaves extracs red betel (P. crocatum) which is antibacterial is a volatile oil, flavonoids, alkaloids and tannins.   The purpose of  this study was  to determine  the Minimum  Inhibitory Concentration  (MIC) and Minimum  Bactericidal  Concentration  (MBC)  of  leaves  extracts  red  betel  (P.  crocatum)  against  of bacteria  A.  hydrophila.  The  research was  conducted  on  July-August  2011,  at  the  faculty  of  veterinary medicine, Airlangga University and Organic Chemistry Laboratory Faculty Of  science and Technology Airlangga  University.  The  method  used  in  this  study  is  an  experimental  method,  using  completely randomized design  (RAL) with 11  treatments and  three  replications. Variables observed  in  this  study  is the minimum  inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of  leaves extracts red betel (P. crocatum). The observations were analyzed using analysis of variance (ANAVA). The results showed that extracts  leaves red betel (P. crocatum) has efficacy against the bacteria A.  hydrophila  in  vitro. Based  on  the  results  of MIC  testing,  leaves  exracts  of  red  betel  (P.  crocatum) inhibits growth of bacteria A. hydrophila at a concentration of 12,5% and based on  test results of MBC, leaves extracs of red betel (P. crocatum) effectively kills the bacteria A. hydrophila at a concentration of 25%. It can be concluded that the concentration of antibacterial substances contained  in red betel able to inhibit and kill bacteria A. hydrophila. This study recommends  further  to determine  the effects of  leaves extracts  red betel (P. crocatum) A. hydrophila against bacteria in vitro, so that can know the effectiveness of  leaves  effects  of  leaves  extracts  red  betel  (P.  crocatum)  in  suppressing  populations  of  bacteria  A. hydrophila.

jurnal ilmiah perikanan dan kelautan/jurnal ilmiah perikanan dan kelautan

Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro &lt;br&gt;&lt;i&gt;[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]&lt;i&gt;