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Background Mineral trioxide aggregate (MTA) and resin modified glass ionomer cement (RMGIC) are the material used for indirect and direct pulp capping due to biocompatibility, but these materials have many shortcomings. Mineral trioxide aggregate contains a little amount of arsenic and has long setting time, while HEMA containing RMGIC are cytotoxic. Rice husk ash nanoparticles (RHAn) is a potential source of silica. High molecular chitosan nanoparticles (HMCn) can stimulate the formation of reparative dentin. Combination of these two materials is biocompatible and have good sealing ability. Purpose This study was aimed to study RHAn + HMCn used as biomaterials for prevention of pulpodentinal complex by examined at the microstructure of dentin surfaces applied with RHAn + HMCn. Methods Twenty-four mandibular premolar teeth extracted for orthodontic purposes, were made cavity class I preparation with 3 mm depth above the cemento-enamel junction (CEJ). Then each tooth was cut in bucco-lingual direction and each part was cut using a cervical disc bur. Samples were divided into 3 groups, group I the teeth were applied with MTA; group II the teeth were applied RMGIC; group III the teeth were apllied with RHAn + HMCn. Characterization was done by using Scanning Electron Microscopy (SEM) on the interface between test material and dentin adjacent to the pulp to see surface microstructure. Results Material microstructure of RHAn + HMCn applied to the dentine showed tags like structure which was more significant than MTA. RHAn + HMCn showed to have better sealing ability than MTA. Porosity of ASPn + HMCn was less than MTA and RMGIC. Conclusion The study suggested that the combined RHAn + HMCn biomaterials could be used as an active biomaterial that can maintain the integrity of pulp dentinal complex.

dental journal

Evaluasi karakteristik abu sekam padi dengan kitosan molekul tinggi nanopartikel sebagai bahan dentinogenesis (Characteristic evaluation of rice husk ash with chitosan high molecule nanoparticle as dentinogenesis material)