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Background: This study was done to assess the effectiveness of removal using chemomechanical agent (Papacarie Duo) and conventional hand excavation methods in primary and permanent molars on the residual cariogenic bacteria, analyzing the time required for the procedure and the need for anesthesia. Materials and Methods: The present in vivo study was conducted 30 children of the age groups between 4 and 8 years. Using a split- mouth design, the teeth were randomly allocated into two groups, Group I - Papacarie Duo and Group II - hand excavation method. The groups were further subdivided into Group IA and Group IIA for primary molars and Group IB and Group IIB for permanent molars. The dental caries samples before and after caries removal from each group were taken to the Department of Microbiology and processed to check for the growth of Streptococcus mutans and Lactobacillus. The colony forming units were counted using a digital colony counter, and the results were subjected to statistical analysis. The degree of discomfort during caries excavation was evaluated and the time taken for complete removal of caries was recorded using a digital stopwatch. Results: The mean total viable count of S. mutans and Lactobacillus considerable reduction after caries removal in both Papacarie Duo and the hand excavation groups. The difference in the mean values for S. mutans between the two groups was found to be statistically significant (P = 0.001). However, the difference was not statistically significant for Lactobacillus (P = 0.91). The mean time required for the Papacarie Duo gel was lesser than hand excavation method. The degree of discomfort was significantly higher in the hand excavation group compared with that of Papacarie Duo group. Conclusion : The chemomechanical method (Papacarie Duo) is more effective in the reduction of both S. mutans and Lactobacillus from the carious dentine of primary and permanent molars than the hand excavation method.

journal of international oral health

Microbiological Assessment of Carious Dentine using Chemomechanical Caries Removal and Conventional Hand Excavation in Primary and Permanent Teeth: A Clinical Study