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El objetivo de este estudio fue evaluar si existe sinergismo antibacteriano sobre Enterococcus faecalis y Pseudomonas aeruginosa, al asociar iodoformo con CaOH2. Para evaluar el efecto antibacteriano de la asociacion experimental del hidroxido de calcio (CaOH2) y el iodoformo sobre Enterococcus faecalis (ATCC 1495) y Pseudomonas aeruginosa (ATCC 9027), fue preparada una suspension padronizada de la bacteria en 108 celulas/mL, que luego fue sembrada,en placas conteniendo agar Muller Hinton. Despues de 10 minutos a 37°C, fueron realizados pozos de 5mm de diametro en el agar, colocando en ellos CaOH2, iodoformo y la asociacion de ambos, utilizando como vehiculos solucion fisiologica, lidocaina al 2% con epinefrina, polietilenglicol 400 y paramonoclorofenol. Se incubaron las placas petri a 37°C durante 48 horas y despues se calcularon los halos de inhibicion de crecimiento bacteriano. Utilizando el test de multiples comparaciones de Tukey, los resultados mostraron que el iodoformo tuvo accion antibacteriana solo cuando fue utilizado el paramonoclorofenol como vehiculo, atribuyendose la accion antibacteriana a este ultimo, siendo esta semejante a la accion antibacteriana mostrada por el hidroxido de calcio puro y en asociacion con el iodoformo (p > 0,05). El iodoformo mostro mayor inhibicion frente a P. aeruginosa en comparacion a la accion mostrada frente a E. faecalis. No hubo diferencia estadisticamente significativa entre la accion antibacteriana del CaOH2 puro y asociado al iodoformo, independientemente del vehiculo utilizado. Se concluye que el efecto antibacteriano del CaOH2 sobre E. faecalis y P. aeruginosa prevalece al mostrado por el iodoformo y que la asociacion de ambos no altera ese resultado.(AU) The aim of this study was to assess whether there is antibacterial synergism on Enterococcus faecalis and Pseudomonas aeruginosa when iodoform was mixed to calcium hydroxide. A bacterial suspension containing 108 cells.ml of Enterococcus faecalis (ATCC 1495) and Pseudomonas aeruginosa (ATCC 9027), were plated on Muller Hinton agar, in duplicate, witha sterile swab. After 10 minutes at 37°C, 4 holes of 5 mm in diameter were prepared in the agar and filled with CaOH2, iodoform and the association of both, mixed with 4 different vehicles: physiologic solution, 2% lidocaine with epinephrine, polyethylene glycol 400 and paramonochloro-phenol. The plates were incubated at 37°C during 48 hours and then the halos demonstrating inhibition of bacterial growth were measured. Using Tukey's test, the results showed that iodoform had antibacterial effect only when used as a vehicle of paramonochlorophenol, being this action attributed to the vehicle, which was similar to the antibacterial effect shown by calcium hydroxide pure and mixed with iodoform (p> 0.05). Iodoform showed greater inhibition against P. aeruginosa compared to E. faecalis. There was no statistically significant difference between the antibacterial effect of CaOH2 pure and mixed with iodoform, regardless the vehicle used. We conclude that the antibacterial effect shown by CaOH2 on E. faecalis and P. aeruginosa prevails over that of iodoform and their association does not alter this result.(AU)

Efecto antibacteriano de la asociación de hidróxido de cálcio y iodoformo sobre Enterococcus faecalis y Pseudomonas aeruginosa