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ultraviolet–diode array detection (UV-DAD) for the simultaneous determination of atrazine, malathion, fenitrothion, and parathion residues in apple juices has been developed. For the enrichment and cleanup of compounds of interest, Supelclean ENVI-18 SPE tubes were used. Separation and quantitative determination of the analytes were performed on a LiChrospher 60 RP-select B (125 mm × 4 mm, 5 μm, Merck) analytical column, the mobile phase consisting of acetonitrile/water (55/45, V/V ) in isocratic elution with the following set values: flow rate of 1 ml/min, constant column temperature at 25 oC, and UV detection at 220 nm and 270 nm. The gathered values of the investigated pesticides from the apple juice samples were in the range 94.2 %–117.2 %.

macedonian journal of chemistry and chemical engineering

Optimization and development of SPE-HPLC-DAD method for the determination of atrazine, malathion, fenitrothion and parathion pesticide residues in apple juice