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Culture dependent methods have been characterized by laborious and time-consuming procedures and allows generally to recovery less than 20-30% of total bacteria richness [2]. The introduction of culture independent methods has allowed to understand and to overcome the limitation of microbial cultivation [3]. It was applied in food microbiology as tools to analyze foodstuff microbiota, to study fermentation and food spoilage processes and also to investigate the ecology of food-borne pathogens [4, 5].

High-Throughput Sequencing Technologies and new Approaches in Food Microbiology