English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Resistance to β-lactams owing to the production of extended-spectrum β-lactamases (ESBLs) enzymes has become common among diarrheagenic E. coli, especially in developing countries. This study aimed to detect ESBLs encoding genes blaTEM, blaCTX-M and blaSHV among E. coli isolates from patients with acute infectious diarrhoea in Cairo, Egypt. A total of 60 E. coli isolates were isolated from diarrhoea stool cultures, during the period from March 2014 to December 2015, at three hospitals in Cairo. Isolation and identification of E. coli were performed using standard microbiological methods and confirmed by Vitek 2 automated system. Antimicrobial susceptibility testing was performed using Kirby-Bauer disc diffusion method against diverse classes of antimicrobial agents. Confirmatory tests for ESBLs production were performed using standard disc diffusion methods according to Clinical and Laboratory Standards Institute (CLSI). The E. coli ESBL-producer phenotypes were investigated for the presence of blaTEM, blaCTX-M and blaSHV via polymerase chain reaction (PCR) using each gene-specific primers. Based on CLSI guidelines of E. coli isolates were multi-drug resistant (MDR) ESBLs-producer. All isolates were resistant to penicillins, thirdand fourth-generation cephalosporins and aztreonam. E. coli isolates showed high resistance rates to non-β-lactams antimicrobial agents. The combination disc method for detection of ESBLs production showed positive results in 86 % of isolates. The cefoxitin-cloxacillin double disc synergy test for AmpC production showed positive results in 18 % of isolates. PCR studies revealed that blaTEM was the predominant ESBLs encoding determinant in 85 % of isolates, followed by blaCTX-M in 73.3 % of isolates; blaSHV was not detected in this study. All ESBLs-producing E. coli isolates possessed one or two of the PCR-positive ESBLs determinants. Each blaTEM and blaCTX-M was found alone in 63.3 % and 51.7 % of all isolates, respectively. The blaTEM in combination with blaCTX-M was detected in 21.7 % of E. coli isolates. ESBLs production is a noteworthy increasing among E. coli causing diarrhoeal diseases. The blaTEM is the common genetic mechanism for ESBL production in these pathogens isolated from Egypt. The limited treatment alternatives of infections caused by ESBL-producers E. coli require a public health policy on appropriate prescribing and the rational use of antimicrobial agents.

international journal of research studies in microbiology and biotechnology

PCR-Based Molecular Detection of ESBLs Encoding Genes blaTEM, blaCTX-M and blaSHV among MDR Escherichia coli Isolates from Diarrhoea Stool Cultures in Cairo, Egypt.