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Thirteen different plant and animal protein media such as nutrient agar medium-I, nutrient agar medium-II, Wouts medium, modified Wouts medium, wheat flour medium, modified wheat flour medium, egg yolk medium-I, egg yolk medium-II, modified egg yolk medium, dog biscuit medium, modified dog biscuit medium, bengal gram medium-I and bengal gram medium-II were tested for mass multiplication of entomopathogenic nematodes such as Heterorhabditis bacteriophora strain KKMH1 and Steinernema carpocapsae strain APKS2 by in vitro solid culture technique. Results showed that modified Wouts medium and nutrient agar medium-I multiplied significantly higher numbers of 39.02 39.14 lakh IJs of H.. bacteriophora strain KKMH1 per 250 ml flask. The IJs produced from these media were more virulent to cotton pest such as Helicoverpa armigera, Earias vitella and Spodoptera litura. For multiplication of Steinernema carpocapsae strain APKS2, modified dog biscuit medium and nutrient agar medium-I got significantly higher IJ populations of 49.69 52.31 lakh /250 ml flask. The IJs multiplied on these media caused 100% mortality of H. armigera, E. vitella and S. litura. The results of this study suggest that mass production of the H. bacteriophora strains KKMH1 on modified wouts medium or nutrient agar medium-I and S. carpocapsae strain APKS2 on modified dog biscuit medium or nutrient agar medium-I are promising option while promotion of these strains as potential bio-pesticide against cotton insect pests H. armigera, E. vitella and S. litura.

international journal of research studies in zoology

Evaluation of Different Solid Media for Mass Production of Native Entomopathogenic Nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae Isolated from Cotton Fields