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Purpose: This study aims to establish the appropriate conditions for induction and proliferation of callus from the flower stalk of  Calanthe discolor  Lindl. (CFS) and to analyze the antioxidant properties as well as the skin irritation level of its extracts.Methods: Murashige &amp; Skoog medium was used to induce the callus from CFS with NB0 (α-naphthaleneacetic acid (NAA) 0 mg/L, benzyl adenine (BA) 0 mg/L), NB1 (NAA 0.2 mg/L, BA 0.1 mg/L), NB5 (NAA 0.2 mg/L, BA 0.5 mg/L), and NB10 (0.2 mg/L, BA 1.0 mg/L) as plant growth regulators (PGR). The callus powder was extracted by ultrasonic treatment, and the total phenolic contents (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and human skin irritation test using callus extracts were performed.Results: As a result of callus induction from CFS with PGR, NB5 showed the highest growth rate of callus with a weight of 4.2±0.3 g. The TPC of the callus extracts NB0, NB1, NB5, and NB10 were 33.71±0.27, 37.59±0.51, 46.26±0.18, and 55.92±0.33 mg GAE/g, respectively. NB10 showed the highest value of DPPH radical scavenging activity than that of NB0, NB1, NB5, and NB10, in various concentrations. CFS callus extract showed very low human skin irritation compared to that of the control group, 10% SLS.Conclusion: The optimal PGR condition for induction of CFS callus was established as NB10. CFS callus extracts can be used as functional materials for cosmetics as it has significant antioxidant activity and low skin irritation value.

Induction and Proliferation of Callus in the Flower Stalk of Calanthe discolor Lindl. and its Antioxidant Properties