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In this project, the viability and adequacy of simultaneous analysis conducted in a single reactional zone of Microfluidic Paper-based Analytical Device (?PAD) though the mixure of 2 or more colorimetric reagents was evaluated for synthetic samples with the concentration range of 0 to 100 ppm for the analytes. The analytical system used in this research was based on a combination of the aluminum chloride method to quantify flavonoids through complex formation and the Folin-Ciocalteu reagent method to quantify the total phenolic content through redox reactions. Both methods were adapted from their original spectrophotometric systems to new microfluidic ones.

Viability of simultaneous analysis through reagent mixture in microfluidic paper-based analytical devices using colorimetric measures