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Cancer is the second cause of death in the world and can affect the host metabolism, increasing the muscle spoliation and decreasing the adipocyte tissue, inducing the cachectic state in these patients. This work aimed to analyse how the factors produced by tumour cells could affect the myotube C2C12 growth or activities when these cells were treated with culture medium from tumour cells; with this procedure, we could mimic the same situation as in cancer patient. The results showed that there is a correlation between the cellular area variation and the time of the treatments, and the W group led to a decreased cell confluence layer, suggesting that the tumour cells medium likely contained some factor that affected the C2C12 growth.

Interection effects betwen C2C12 and Walker 256 cells