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According to the Centers for Sterilization of Dental-Medical-Hospital Materials, the development of effective cleaning products in the removal of microbial load has been of the utmost importance. The addition of enzymes to these products promotes a beneficial effect, but different effects have been observed depending on the biofilm and the enzymes evaluated. The use of biosurfactants in therapeutic applications aims to inhibit bacterial adhesion and the formation of biofilms. Thus, the objective of this work was to evaluate the efficiency of these cleaning agents containing enzymes of the class of proteases and biossurfactant surfactin in the removal of bacterial biofilm of Escherichia coli under different concentration and time conditions.

Removal of bacterial biofilm from Escherichia coli by synergistic action of serine proteases and biosurfactant