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Quantification of metabolites in blood serum is a challenge due to the presence of endogenous compounds in the sample matrix. Therefore, a thorough evaluation for method validation is required. In this work, different matrices were evaluated: synthetic serum, blood serum charcoal stripped and blood serum with standards addition. Matrix effect was assessed to determine the best method for quantification of endogenous metabolites.

Evaluation of methods for quantification of endogenous metabolites in blood serum: application in the study of potential tumor biomarkers