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The present study was designed to investigate the pathology of bovine rotavirus A infection in dead calves with the history of diarrhea. The cases were further confirmed by ELISA and RT-PCR. A total of 7 dead calves were examined by recommended post mortem techniques followed by confirmatory diagnostic tools. Four calves were found positive by both ELISA and RT-PCR assays. Grossly, no characteristic pathological lesions were observed except congestion in small intestine. The characteristic histopathological lesions were observed mainly in jejunum, ileum, Peyer’s patches and mesenteric lymphnode. Blunting of villi, lymphocytic infiltration, intracytoplasmic eosinophilic inclusion body, nuclear degradation and syncytia formation were observed under the microscope with different magnification. However, observation of pathological changes is important for identification rotaviral infection in case of doubtful specimens. To our knowledge, this is the first report from Bangladesh applying post-mortem examination, ELISA and RT-PCR for detection of bovine rotavirus group A from diarrheic dead calves.

Histopathology in Bovine Rotavirus (Type A) Infected Calves and Its Confirmation by ELISA and RT-PCR