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Vitamins are organic compound (non-energy producing), which are essential for normal human metabolism that must be supplied in small quantities in the diet. The definition excludes the inorganic essential trace minerals and essential amino acids and fatty acids which are required in much larger quantities. Other substances needed for proper growth of microorganism or cells in culture are called growth factors. The different chemical forms and precursors of vitamin can be called its vitamers. The vitamins as a drug is necessary in the prevention and treatment of deficiency disease. Some vitamins do have major uses in pharmacological uses in pharmacological doses. Vitamin deficiency occur due to inadequate intake, malabsorption, increased tissue needs, increased excretion, certain genetic abnormalities and drug vitamin interaction. Vitamin C is a major water-soluble antioxidant. Generally, vitamin C is 6 carbon organic acid with structural similarity to glucose. It acts as a potent reducing agent and its l form is generally more active. Citrus fruits are rich sources of vitamin C. According to recent research human milk contains more amount of vitamin C than the cow’s milk. It is mostly absorbed from g.i.t and widely distributed extra and intra muscularly. Plasma concentration and total body store of vitamin c depends upon daily intake of ascorbic acid content food. The usual 60mg/ day intake results in about 0.8mg/dl in plasma and 1.5g in the body as a whole. Increasing proportion are excreted in urine with higher intake. Body is not able to store more than 2.5g. It is partly oxidised to active (dehydroascorbic acid) and inactive (oxalic acid) metabolites. The therapeutic uses of ascorbic acid include prevention of ascorbic acid deficiency in patient at a risk and in infants, treatment of scurvy, in anaemia, to acidify the urine in urinary tract infection. They lower blood pressure and cholesterol level in the body. Also reduces the cold and has beneficial effect in the bacterial infection. Adequate amount of vitamin c intake may also prevent developing cancer of breast, cervix, colon etc.

UV Spectroscopic Method for Determination of Vitamin C (Ascorbic Acid) Content in Different Fruits in South Gujarat Region